Primer

Part:BBa_K4927012:Design

Designed by: Chuan Liu   Group: iGEM23_HUBU-SKY-China   (2023-10-06)


CL2-F


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Note that the primer length should be controlled around 25bp, and attention should also be paid to the annealing temperature



Source

It is designed by ourselves.

References